Clinical Applications of Mass Spectrometry in Biomolecular by Uttam Garg

By Uttam Garg

This quantity offers stepwise directions for the research of diverse clinically vital analytes by means of mass spectrometry. Mass spectrometry deals medical laboratory scientists a number of benefits together with elevated sensitivity and specificity, a number of part research, and little need for specialised reagents. The innovations defined are a needs to for the dimension of many clinically suitable analytes within the fields of drug research, endocrinology, and inborn error of metabolism. every one bankruptcy presents a quick advent a few particular analyte, through unique directions at the analytical protocol. Written within the hugely profitable Methods in Molecular Biology series layout, chapters contain introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, without difficulty reproducible laboratory protocols, and tips about troubleshooting and warding off recognized pitfalls.

Cutting aspect and functional, Clinical purposes of Mass Spectrometry in Biomolecular research: tools and Protocols is a brilliant source for medical laboratory scientists who're already utilizing or taking into account bringing mass spectrometry to their laboratories.

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Extra resources for Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols

Sample text

Naylor EW, Chace DH (1999) Automated tandem mass spectrometry for mass newborn screening for disorders in fatty acid, organic acid, and amino acid metabolism. J Child Neurol 14(Suppl 1):S4–S8 2. Rinaldo P, Cowan TM, Matern D (2008) Acylcarnitine profile analysis. Genet Med 10: 151–156 3. Schmidt-Sommerfeld E, Penn D, Duran M, Bennett MJ, Santer R, Stanley CA (1993) Detection of inborn errors of fatty acid oxidation from acylcarnitine analysis of plasma and blood spots with the radioisotopic exchange-highperformance liquid chromatographic method.

Opened calibrator vials are for one time use only. Opened quality control vials are stable for 7 days at −20 °C. 3. S. solution is pre-aliquoted and stored in −70 °C until use. 0-mL cryogenic vials. 30 Faye B. Vicente et al. Opened vials are for one time use only. When more than one vial of the solution is required to prepare a batch of samples, combine and mix well before use. 4. Charcoal dextran stripped serum is preferred for preparing quality controls because it contains minimal level of endogenous ADMA, SDMA, and arginine.

Owen WE, Roberts WL (2005) Performance characteristics of an HPLC assay for urinary albumin. Am J Clin Pathol 124:219–225 5. Busby DE, Bakris GL (2004) Comparison of commonly used assays for the detection of microalbuminuria. J Clin Hypertens (Greenwich) 6:8–12 6. Sviridov D, Meilinger B, Drake SK, Hoehn GT, Hortin GL (2006) Coelution of other proteins with albumin during size-exclusion HPLC: implications for analysis of urinary albumin. Clin Chem 52:389–397 7. Singh R, Crow FW, Babic N, Lutz WH, Lieske JC, Larson TS, Kumar R (2007) A liquid chromatography-mass spectrometry method for the quantification of urinary albumin using a novel 15N-isotopically labeled albumin internal standard.

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